Synchronous long-term oscillations in a synthetic gene circuit
نویسندگان
چکیده
منابع مشابه
Mesoscopic oscillations in a single-gene circuit without delay
It is well known that single-gene circuits with negative feedback loop can lead to oscillatory gene expression when they operate with time delay. In order to generate these oscillations many processes can contribute to properly timing such delay. Here we show that the time delay coming from the transitions between internal states of the cis-regulatory system (CRS) can drive sustained oscillatio...
متن کاملCorrelated Noise in a Synthetic Gene Circuit Reveals Regulation
Simulations and analysis in the previous chapter are based on parameters for the λ cI system. Here, we test those predictions experimentally by building a synthetic circuit using the λ cI repressor and other characterized circuit elements in E. coli bacteria. The synthetic circuit uses three fluorescent proteins so that noisy gene expression can be monitored with time-lapse microscopy. To apply...
متن کاملGenome engineering using a synthetic gene circuit in Bacillus subtilis
Genome engineering without leaving foreign DNA behind requires an efficient counter-selectable marker system. Here, we developed a genome engineering method in Bacillus subtilis using a synthetic gene circuit as a counter-selectable marker system. The system contained two repressible promoters (B. subtilis xylA (Pxyl) and spac (Pspac)) and two repressor genes (lacI and xylR). Pxyl-lacI was inte...
متن کاملA synthetic gene circuit for measuring autoregulatory feedback control.
Autoregulatory feedback loops occur in the regulation of molecules ranging from ATP to MAP kinases to zinc. Negative feedback loops can increase a system's robustness, while positive feedback loops can mediate transitions between cell states. Recent genome-wide experimental and computational studies predict hundreds of novel feedback loops. However, not all physical interactions are regulatory,...
متن کاملMapping the Environmental Fitness Landscape of a Synthetic Gene Circuit
Gene expression actualizes the organismal phenotypes encoded within the genome in an environment-dependent manner. Among all encoded phenotypes, cell population growth rate (fitness) is perhaps the most important, since it determines how well-adapted a genotype is in various environments. Traditional biological measurement techniques have revealed the connection between the environment and fitn...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nature
سال: 2016
ISSN: 0028-0836,1476-4687
DOI: 10.1038/nature19841